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13 and hepatocellular carcinoma. 14,15 In a previous analysis of the lncRNA expression profile using a microarray assay, we found that AFAP1-AS1 was upregulated in six GC tissues compared with paired non-cancerous tissues. To investigate the relationship between AFAP1-AS1 and GC, the expression of AFAP1-AS1 was further verified in a study involving a larger sample size composed of tissues and six different GC cell lines. In addition, the potential functions of AFAP1-AS1 on the proliferation, migration, and invasion of GC cells was also investigated in this study. Materials and Methods Tissue Samples and RNA Extraction In this investigation, 97 fresh frozen GC and their paired non-cancerous tissues were collected at Fuzhou General Hospital, which were used to assess the expression of AFAP1-AS1. The collection and preservation of fresh GC tissue samples were carried out as previously described. ....
Hepatocyte TLR4 triggers inter-hepatocyte Jagged1/Notch signaling to determine NASH-induced fibrosis sciencemag.org - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from sciencemag.org Daily Mail and Mail on Sunday newspapers.
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Yuwen Han, Jun You, Yun Han, Yinglei Liu, Menghui Huang, Xiaoyan Lu, Jingjing Chen, Yanli Zheng Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People’s Republic of China Correspondence: Yanli Zheng Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nantong University, No. 6 North Hai-Er-Xiang Road, Chongchuan District, Nantong, 226001, Jiangsu Province, People’s Republic of China Tel +86-0513-85061000 Objective: Cisplatin resistance is one of the main reasons for treatment failure in ovarian cancer (OC). Here, the effects of LINC00184 on cisplatin-resistant OC were studied. Patients and Methods: LINC00184, miR-1305 and CNTN1 expression in tissues from 70 OC patients was determined by qRT-PCR, in situ hybridization and Western blot. OC cell lines and OC cisplatin-resistant cell lines were cultured. Cells were transfected using Lipofectamine 2000 an ....