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LncRNA AFAP1-AS1 Modulates Gastric Cancer Cell Proliferation


13 and hepatocellular carcinoma.
14,15 In a previous analysis of the lncRNA expression profile using a microarray assay, we found that AFAP1-AS1 was upregulated in six GC tissues compared with paired non-cancerous tissues. To investigate the relationship between AFAP1-AS1 and GC, the expression of AFAP1-AS1 was further verified in a study involving a larger sample size composed of tissues and six different GC cell lines. In addition, the potential functions of AFAP1-AS1 on the proliferation, migration, and invasion of GC cells was also investigated in this study.
Materials and Methods
Tissue Samples and RNA Extraction
In this investigation, 97 fresh frozen GC and their paired non-cancerous tissues were collected at Fuzhou General Hospital, which were used to assess the expression of AFAP1-AS1. The collection and preservation of fresh GC tissue samples were carried out as previously described. ....

Eteläuomen Läi , United States , Trizol Invitrogen , Shanghai Institute Of Biochemistry , Cancer Institute , Cell Bank , Chinese Academy Of Sciences , Mix Red Ribobio Co Ltd , Ethics Committee , Fuzhou General Hospital , Joint Logistics Team Hospital , Expression Profile , Kangchen Biotech , Beijing Cancer Institute , Shanghai Institute , Cell Biology , Chinese Academy , Time Quantitative Polymerase Chain Reaction , Mix Kit , Primer Sequences , Cell Counting , Molecular Devices , Cell Light Edu , Proliferation Kit , Apollo Dye , Blotting Assay ,

4D pharma : Annual Report (SEC Filing - 20-F)



Not for trading, but in connection with the registration of American Depositary Shares, pursuant to the requirements of the Securities and Exchange Commission.
Securities registered or to be registered pursuant to Section 12(g) of the Act:
None
None
Indicate the number of outstanding shares of each of the issuer s classes of capital or common stock as of the close of the period covered by the annual report:
Title of each class
Ordinary Shares, nominal value £0.0025 per
Share
131,467,935
Indicate by check mark if the registrant is a well-known seasoned issuer, as defined in Rule 405 of the Securities Act.
Yes [ ] No [X] ....

United States , Hong Kong , District Of Columbia , United Kingdom , State Of New York , New York , New Zealand , British Virgin Islands , South Korea , South Africa , Palm Beach Gardens , University Of San Diego , United Arab Emirates , Northern Ireland , Nordrhein Westfalen , City Of , Saudi Arabia , Duncan Peyton , D Pharma Le , John Beck , Antonio Fernandez , Merck Kga , Edgardo Baracchini , Ardea Biosciences , Merck Serono , Sandy Macrae ,