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The adc1 knockout with proC overexpression in Synechocystis sp. PCC 6803 induces a diversion of acetyl-CoA to produce more polyhydroxybutyrate | Biotechnology for Biofuels and Bioproducts

Lack of nutrients, in particular nitrogen and phosphorus, has been known in the field to sense glutamate production via 2-oxoglutarate and subsequently accelerate carbon storage, including glycogen and polyhydroxybutyrate (PHB), in cyanobacteria, but a few studies have focused on arginine catabolism. In this study, we first time demonstrated that gene manipulation on proC and adc1, related to proline and polyamine syntheses in arginine catabolism, had a significant impact on enhanced PHB production during late growth phase and nutrient-modified conditions. We constructed Synechocystis sp. PCC 6803 with an overexpressing proC gene, encoding Δ1pyrroline-5-carboxylate reductase in proline production, and adc1 disruption resulted in lower polyamine synthesis. Three engineered Synechocystis sp. PCC 6803 strains, including a ProC-overexpressing strain (OXP), adc1 mutant, and an OXP strain lacking the adc1 gene (OXP/Δadc1), certainly increased the PHB accumulation under nitrogen and ....

Baden Wüberg , United States , United Kingdom , King Lynn , Gmbh Co , Labconco Corporation , Life Technologies Corporation , Co Ltd , Agilent Technologies , Carl Zeiss , C Overexpressing Synechocystis , Mix Kit , Gel Documentation , Agilent Zorbax Eclipse ,

Vinyard Fruit and Vegetable Company Recalls Cantaloupe Due to Potential Salmonella Contamination

Vinyard Fruit and Vegetable Company, located in Oklahoma City, OK, has initiated a voluntary recall of all fresh-cut cantaloupe products listed below because they have the potential to be contaminated with Salmonella, an organism which can cause serious and sometime fatal infections in young childre ....

United States , Oklahoma City , Jennifer Vogler , Company Contact Information , Company Name , Canadian Food Inspection Agency , Vegetable Company , Company Announcement , Sophia Foods , Announcement Date , Fruit Mixes , Medleys Containing , Immediate Release , Vinyard Fruit , Medley Cup , Mix Kit ,