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TAT&RGD peptide-modified naringin-loaded lipid nanoparticles

We prepared TAT-RGD-NAR-NPs, evaluated their potency on the osteogenic differentiation of hDPSCs, & studied its mechanism of action through metabolomic analysis ....

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Targeted delivery of nanovaccine to dendritic cells

Targeted delivery of nanovaccine to dendritic cells via DC-binding peptides induces potent antiviral immunity in vivo ....

Laboratory Animal Care , Guideline For Ethical Review Of Laboratory Animal , Zhejiang University , Guiding Opinions On Treating Laboratory Animals , Laboratory Animal Management Regulations , Genbank Accession Number , Murine Bone Marrow Derived Dendritic Cells , Marker Genes , Cytokine Expression , Chamq Universal , Primer Sequences , Use Committee , Ethical Review , Laboratory Animal , Guiding Opinions , Treating Laboratory Animals Kindly , Cap Specific Igg , Fusion Proteins , Cellular Uptake ,

LncRNA AFAP1-AS1 Modulates Gastric Cancer Cell Proliferation

13 and hepatocellular carcinoma.
14,15 In a previous analysis of the lncRNA expression profile using a microarray assay, we found that AFAP1-AS1 was upregulated in six GC tissues compared with paired non-cancerous tissues. To investigate the relationship between AFAP1-AS1 and GC, the expression of AFAP1-AS1 was further verified in a study involving a larger sample size composed of tissues and six different GC cell lines. In addition, the potential functions of AFAP1-AS1 on the proliferation, migration, and invasion of GC cells was also investigated in this study.
Materials and Methods
Tissue Samples and RNA Extraction
In this investigation, 97 fresh frozen GC and their paired non-cancerous tissues were collected at Fuzhou General Hospital, which were used to assess the expression of AFAP1-AS1. The collection and preservation of fresh GC tissue samples were carried out as previously described. ....

Eteläuomen Läi , United States , Trizol Invitrogen , Shanghai Institute Of Biochemistry , Cancer Institute , Cell Bank , Chinese Academy Of Sciences , Mix Red Ribobio Co Ltd , Ethics Committee , Fuzhou General Hospital , Joint Logistics Team Hospital , Expression Profile , Kangchen Biotech , Beijing Cancer Institute , Shanghai Institute , Cell Biology , Chinese Academy , Time Quantitative Polymerase Chain Reaction , Mix Kit , Primer Sequences , Cell Counting , Molecular Devices , Cell Light Edu , Proliferation Kit , Apollo Dye , Blotting Assay ,

Inhibition of Long Non-Coding RNA KCNQ1OT1 Attenuates Neuroinflammatio

Knocking Down KCNQ1OT1 Suppressed LPS-Induced Neuroinflammation and Neuronal Apoptosis in HMC3 Cells
For further exploring the function of KCNQ1OT1, we transfected HMC3 cells with pc-KCNQ1OT1 or si-KCNQ1OT1. The expression of KCNQ1OT1 was evaluated by RT-qPCR. Compared with control cells (cells transfected with pc-NC or si-NC), the expression of KCNQ1OT1 in HMC3 cells transfected with pc-KCNQ1OT1 was significantly increased, while the expression of KCNQ1OT1 in cells transfected with si-KCNQ1OT1 was significantly decreased (Figure 2A). Subsequently, we used RT-qPCR and ELISA kits to examine the expressions of TNF-α, IL-1β and IL-6 to evaluate the inflammatory response. The results indicated that LPS treatment markedly promoted the expression levels of inflammatory cytokines TNF-α, IL-1β and IL-6 in HMC3 cells; overexpression of KCNQ1OT1 promoted the expressions of inflammatory cytokines, while KCNQ1OT1 knockdown inhibited their expression (Figure 2B and C). The expression o ....

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Hsa_circ_0000144 regulates miR-502-5p/ADAM9 axis

Hsa_circ_0000144 regulates miR-502-5p/ADAM9 axis
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