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A spatially resolved timeline of the human maternal–fetal interface

Beginning in the first trimester, fetally derived extravillous trophoblasts (EVTs) invade the uterus and remodel its spiral arteries, transforming them into large, dilated blood vessels. Several mechanisms have been proposed to explain how EVTs coordinate with the maternal decidua to promote a tissue microenvironment conducive to spiral artery remodelling (SAR)1–3. However, it remains a matter of debate regarding which immune and stromal cells participate in these interactions and how this evolves with respect to gestational age. Here we used a multiomics approach, combining the strengths of spatial proteomics and transcriptomics, to construct a spatiotemporal atlas of the human maternal–fetal interface in the first half of pregnancy. We used multiplexed ion beam imaging by time-of-flight and a 37-plex antibody panel to analyse around 500,000 cells and 588 arteries within intact decidua from 66 individuals between 6 and 20 weeks of gestation, integrating this dataset ....

Leica Biosystems , Dako Agilent , Nanostring Geomx , Flowsom Bioconductor Flow , Perivascular Evts , Electron Microscopy Sciences , Lab Vision Pt Module Thermo Fisher Scientific , Sigma Aldrich , Institutional Review Board At Stanford University , Fisher Scientific , Stanford University , Beckman Coulter , Thermo Fisher , Supplementary Table , Stanford Tissue Microarray Database , Supplementary Information , Candor Bioscience , Thermo Fisher Scientific , Flab Vision , Cell Marque , Biotin Blocking , Deepcell Label , Vector Laboratories , Vector Blue , Nanozoomer Digital Pathology Scanner , Extended Data ,