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Seres Therapeutics Appoints Genomic Medicine and Microbiology Pioneer Claire M. Fraser, Ph.D., to its Board of Directors and Announces SER-155 Phase 1b Study Progress - read this article along with other careers information, tips and advice on BioSpace ....
Cell Lines ESCC cell lines (Eca109, KYSE150, KYSE180 and KYSE450) and human esophageal epithelial cell line HET-1A were purchased from the Shanghai Institute of Chinese Academy of Sciences Cell Collection (Shanghai, China). All these five cell lines were cultured with Roswell Park Memorial Institute-1640 medium (RPMI-1460; Gibco, Carlsbad, CA, USA) added with 10% fetal bovine serum (FBS; Gibco), 1% 100 units/mL penicillin (Gibco) and 1% 100 μg/mL streptomycin (Gibco). All cell lines were maintained at 37°C incubator with 5% CO 2. Western Blot Assay ESCC cells were collected and lysed using whole cell lysis buffer (Abcam). Protein quantification was conducted with the commercial bicinchoninic acid (BCA) protein assay kit (Invitrogen, Carlsbad, CA, USA). Proteins were separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel and transferred onto the polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, MA, USA). After block ....
XYS Activates the PI3K/Akt Pathway Network pharmacology suggested that the PI3k/Akt pathway was the main mechanism of action in IS treatment by XYS. Therefore, we evaluated the effect of XYS on the mRNA and protein expression of PI3k and Akt in PC12 cells. OGD/R inhibited mRNA expression of PI3K and Akt significantly in PC12 cells (Figures 9 and 10). Compared with the normal group, although protein expression of PI3K and Akt did not change significantly, phosphorylation of PI3K and Akt was inhibited significantly. Fortunately, XYS therapy could reverse this inhibition. These results indicated that the PI3k/Akt pathway was involved in the anti-IS action of XYS. ....
25 Nevertheless, the reports of ITGA9 in melanoma are few. The objective of our present study was to explore the role of HOXA11-AS in melanoma and the regulatory mechanism among HOXA11-AS, miR-152-3p and ITGA9 in cellular processes, intending to promote the understanding of the molecular pathogenesis in melanoma. Materials and Methods Ethic Statement For clinical human samples, our study was performed after obtaining ratification from the Ethics Committee of The First Affiliated Hospital of Zhengzhou University. The research has been carried out in accordance with the World Medical Association Declaration of Helsinki, and that all subjects provided written informed consent. The animal experiment in this research was authorized by the Experimental Animal Ethics Committee of The First Affiliated Hospital of Zhengzhou University. Animal studies were performed in compliance with the ARRIVE guidelines and the Basel Declaration. All animals received humane care according ....