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[Full text] MiR-129-5p suppresses cell proliferation via lncRNA Lnc712


Yifan Yu, Wenshan Zuo, Wei Cai, Yong Xu, Weidong Liu, Zexue Zhao
Department of Orthopaedics, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Huai’an City, Nanjing Province, 223300, People’s Republic of China
Correspondence: Wenshan Zuo
Department of Orthopaedics, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, No. 1 Huanghe West Road, Huaiyin District, Huai’an City, Nanjing Province, 223300, People’s Republic of China
Introduction: Lnc712 has been characterized as an oncogenic lncRNA in breast cancer. This study aimed to investigate the role of Lnc712 in osteosarcoma (OS).
Methods: OS and paired non-tumor tissues were collected from 58 OS patients. Expression of Lnc712 and miR-129-5p in paired tissue samples was determined by RT-qPCR. Lnc712 and miR-129-5p expression was achieved in OS cells to study the interaction between them. Cell proliferation was analyzed by CCK-8 assay. ....

Eteläuomen Läi , United States , Dnasei Invitrogen , People Hospital Of Nanjing Medical University , Ethics Committee , Promega Corporation , Nanjing Medical , Reverse Transcription System , Master Mix , Cell Counting Kit , May Target Lnc , ஒன்றுபட்டது மாநிலங்களில் , மக்கள் மருத்துவமனை ஆஃப் நான்ஜிங் மருத்துவ பல்கலைக்கழகம் , நெறிமுறைகள் குழு , ப்ரொமெகா நிறுவனம் , நான்ஜிங் மருத்துவ , தலைகீழ் படியெடுத்தல் அமைப்பு , குரு கலவை , செல் எண்ணும் கிட் , இருக்கலாம் இலக்கு ழ்ந்க் ,

[Full text] CircSKA3 Downregulates miR-1 Through Methylation in Glioblastoma to Pr


1
1Department of Neurosurgery, The First Affiliated Hospital of Jinan University, Guangzhou City, Guangdong Province 510630, People’s Republic of China;
2Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, People’s Republic of China
Correspondence: Xiangyu Wang
Department of Neurosurgery, The First Affiliated Hospital of Jinan University, No. 613 West Huangpu Avenue, Tianhe District, Guangzhou City, Guangdong Province 510630, People’s Republic of China
Background: Circular RNA circSKA3 plays an oncogenic role in breast cancer, while its role in glioblastoma (GBM) is unknown. This study aimed to explore the role of circSKA3 in GBM.
Methods: Differential expression of circSKA3 and miR-1 in GBM and adjacent non-cancer tissue samples were analyzed by RT-qPCR. GBM cells were transfected with circSKA3 expression vector or miR-1 mimic, followed by RT-qPCR to explore the potential crosstalk between them. Methylat ....

Eteläuomen Läi , United States , Dnasei Invitrogen , Ethics Committee , Promega Corporation , First Affiliated Hospital Of Jinan University , First Affiliated Hospital , Jinan University , Reverse Transcription System , Master Mix , Sera Xtracta Genomic , Counting Kit , Prognostic Values , Expression Levels , Proliferation Through , ஒன்றுபட்டது மாநிலங்களில் , நெறிமுறைகள் குழு , ப்ரொமெகா நிறுவனம் , முதல் இணைந்த மருத்துவமனை ஆஃப் ஜினன் பல்கலைக்கழகம் , முதல் இணைந்த மருத்துவமனை , ஜினன் பல்கலைக்கழகம் , தலைகீழ் படியெடுத்தல் அமைப்பு , குரு கலவை , எண்ணும் கிட் , வெளிப்பாடு நிலைகள் ,