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Differential paired stage-specific expression of Babesia bovis cysteine-rich GCC2/GCC3 domain family proteins (BboGDP) during development within Rhipicephalus microplus | Parasites & Vectors

Babesia bovis, an intra-erythrocytic apicomplexan parasite, is one of the causative agents of bovine babesiosis, the most important tick-borne disease of cattle in tropical and subtropical regions. Babesia bovis has a complex life-cycle that includes sexual development within the tick vector. The development of a transmission blocking vaccine to control bovine babesiosis requires the identification of antigens displayed on the surface of the parasite during its development within tick vectors. Four B. bovis cysteine-rich GCC2/GCC3 domain protein (BboGDP) family members were previously identified and are differentially expressed as discrete pairs by either blood stages or kinetes. In this study we focused on two family members, BboGDP1 and -3, that are expressed by Babesia parasites during tick infection. Transcription analysis using quantitative PCR demonstrated that BboGDP1 and -3 were upregulated in in vitro-induced sexual stage parasites and during parasite development in the tick m ....

United States , Microsystems Gmb , Leica Microsystems Gmb , Dnasei Invitrogen , Biosynthesis Inc , University Of Idaho Institutional Animal Care , Electron Microcopy Sciences , Idaho Institutional Animal Care , Use Committee , Thermo Fisher Scientific , Bio Rad Laboratories , Thermal Cycler , Supermix Kit , Alexa Fluor , Leica Microsystems Gmbh , Igg Alexa Fluor , Microsystems Gmbh ,

CircRNA circ_POLA2 is upregulated in acute myeloid leukemia

Hong Li, Kehong Bi, Saran Feng, Yan Wang, Chuansheng Zhu
Department of Hematology, Shandong Qianfoshan Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250014, China
Correspondence: Kehong Bi
Department of Hematology, Shandong Qianfoshan Hospital, Cheeloo College of Medicine, Shandong University, No. 16766 Jingshi Road, Jinan City, Shandong Province, 250014, China
Purpose: CircRNA circ POLA2 has been reported as an oncogene in lung cancer, while its role in other malignancies is unknown. This study aimed to explore the role of circ POLA2 in acute myeloid leukemia (AML).
Methods: The expression levels of circ POLA2, mature miR-34a and miR-34a precursor in bone marrow mononuclear cells (BMMNCs) from AML patients (n = 50) and healthy controls (n = 50) were determined by RT-qPCR. Correlations among them were analyzed by Pearson’s correlation coefficient. Overexpression of circ POLA2 was achieved in AML cell lines, followed by the meas ....

Eteläuomen Läi , United States , Dnasei Invitrogen , Ethics Committee Of Shandong Qianfoshan Hospital , Cell Signaling Technology , Ethics Committee , Shandong Qianfoshan , Master Mix , ஒன்றுபட்டது மாநிலங்களில் , செல் சமிக்ஞை தொழில்நுட்பம் , நெறிமுறைகள் குழு , குரு கலவை ,

[Full text] MiR-129-5p suppresses cell proliferation via lncRNA Lnc712

Yifan Yu, Wenshan Zuo, Wei Cai, Yong Xu, Weidong Liu, Zexue Zhao
Department of Orthopaedics, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Huai’an City, Nanjing Province, 223300, People’s Republic of China
Correspondence: Wenshan Zuo
Department of Orthopaedics, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, No. 1 Huanghe West Road, Huaiyin District, Huai’an City, Nanjing Province, 223300, People’s Republic of China
Introduction: Lnc712 has been characterized as an oncogenic lncRNA in breast cancer. This study aimed to investigate the role of Lnc712 in osteosarcoma (OS).
Methods: OS and paired non-tumor tissues were collected from 58 OS patients. Expression of Lnc712 and miR-129-5p in paired tissue samples was determined by RT-qPCR. Lnc712 and miR-129-5p expression was achieved in OS cells to study the interaction between them. Cell proliferation was analyzed by CCK-8 assay. ....

Eteläuomen Läi , United States , Dnasei Invitrogen , People Hospital Of Nanjing Medical University , Ethics Committee , Promega Corporation , Nanjing Medical , Reverse Transcription System , Master Mix , Cell Counting Kit , May Target Lnc , ஒன்றுபட்டது மாநிலங்களில் , மக்கள் மருத்துவமனை ஆஃப் நான்ஜிங் மருத்துவ பல்கலைக்கழகம் , நெறிமுறைகள் குழு , ப்ரொமெகா நிறுவனம் , நான்ஜிங் மருத்துவ , தலைகீழ் படியெடுத்தல் அமைப்பு , குரு கலவை , செல் எண்ணும் கிட் , இருக்கலாம் இலக்கு ழ்ந்க் ,

[Full text] CircSKA3 Downregulates miR-1 Through Methylation in Glioblastoma to Pr

1
1Department of Neurosurgery, The First Affiliated Hospital of Jinan University, Guangzhou City, Guangdong Province 510630, People’s Republic of China;
2Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, People’s Republic of China
Correspondence: Xiangyu Wang
Department of Neurosurgery, The First Affiliated Hospital of Jinan University, No. 613 West Huangpu Avenue, Tianhe District, Guangzhou City, Guangdong Province 510630, People’s Republic of China
Background: Circular RNA circSKA3 plays an oncogenic role in breast cancer, while its role in glioblastoma (GBM) is unknown. This study aimed to explore the role of circSKA3 in GBM.
Methods: Differential expression of circSKA3 and miR-1 in GBM and adjacent non-cancer tissue samples were analyzed by RT-qPCR. GBM cells were transfected with circSKA3 expression vector or miR-1 mimic, followed by RT-qPCR to explore the potential crosstalk between them. Methylat ....

Eteläuomen Läi , United States , Dnasei Invitrogen , Ethics Committee , Promega Corporation , First Affiliated Hospital Of Jinan University , First Affiliated Hospital , Jinan University , Reverse Transcription System , Master Mix , Sera Xtracta Genomic , Counting Kit , Prognostic Values , Expression Levels , Proliferation Through , ஒன்றுபட்டது மாநிலங்களில் , நெறிமுறைகள் குழு , ப்ரொமெகா நிறுவனம் , முதல் இணைந்த மருத்துவமனை ஆஃப் ஜினன் பல்கலைக்கழகம் , முதல் இணைந்த மருத்துவமனை , ஜினன் பல்கலைக்கழகம் , தலைகீழ் படியெடுத்தல் அமைப்பு , குரு கலவை , எண்ணும் கிட் , வெளிப்பாடு நிலைகள் ,