Investigation of the RNA N6-methyladenosine (m6A) modification in the progression of multiple tumours the tumour microenvironment in non-small cell lung cancer
Yifan Yu, Wenshan Zuo, Wei Cai, Yong Xu, Weidong Liu, Zexue Zhao
Department of Orthopaedics, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, Huai’an City, Nanjing Province, 223300, People’s Republic of China
Correspondence: Wenshan Zuo
Department of Orthopaedics, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University, No. 1 Huanghe West Road, Huaiyin District, Huai’an City, Nanjing Province, 223300, People’s Republic of China
Introduction: Lnc712 has been characterized as an oncogenic lncRNA in breast cancer. This study aimed to investigate the role of Lnc712 in osteosarcoma (OS).
Methods: OS and paired non-tumor tissues were collected from 58 OS patients. Expression of Lnc712 and miR-129-5p in paired tissue samples was determined by RT-qPCR. Lnc712 and miR-129-5p expression was achieved in OS cells to study the interaction between them. Cell proliferation was analyzed by CCK-8 assay.