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Automation of the Agilent SureSelect XT Library Prep Kit with the Human All Exon V6 Panel on the Biomek NGeniuS Next Generation Library Prep System

In the past three decades, methods for sequencing nucleic acids have improved significantly in speed and
accuracy. Despite the technological advances in sequencing, preparation of libraries for Next-generation
sequencing (NGS) remains a tedious process. In this application note, Beckman Coulter Life Sciences demonstrates that the automated processing on the Biomek NGeniuS Next
Generation Library Prep System is equivalent to the manual preparations at input concentration of
200 ng (g ....

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Clinical Implications of a New DDX58 Pathogenic Variant That... : Journal of the American Society of Nephrology

Clinical Implications of a New DDX58 Pathogenic Variant That... : Journal of the American Society of Nephrology
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Diagnosis of Legionnaires' Disease

Metagenomics is a promising approach that facilitated the diagnosis of Legionnaires’ disease ....

United States , Santa Clara , Agilent Technologies , Library Prep Kit , Tissue Kit , Thermo Fisher , Prep Kit , About Legionnaire ,

Antiviral action of Oridonin against herpes simplex virus 1

Oridonin has potential promise for application in treating Herpes Simplex Keratosis and other diseases caused by HSV-1 infection. ....

United States , San Diego , Foster City , Carl Zeiss Meditec , Excell Bio Hongkong , Committee Of Shandong Eye Institute , Shanghai Genechem Co , Weitong Lihua Limited Company , Ethics Committee , Shandong Eye Institute , Shandong First Medical University , Med Chem Express , Excell Bio , Shandong Eye , Shanghai Genechem , Applied Biosystems , Animal Protection , Use Committee , Shandong First Medical , Alexa Fluor , Beyotime Biotechnology , Library Prep Kit , Illumina Hiseq , Thermo Scientific , Truseq Stranded , Prep Kit ,

Frontiers | High-throughput sequencing reveals the presence of novel and known viruses in diseased Paris yunnanensis

Viral diseases of Paris spp. have become serious problems in cultivation of this group of important medicinal plants in China. In this study, eight viruses were identified in the diseased plants of Paris yunnanensis by high-throughput sequencing (HTS) and RT-PCR. These viruses include three novel viruses (two potyviruses and one nepovirus), Hippeastrum chlorotic ringspot virus (HCRV)，Lychnis mottle virus (LycMoV), Paris mosaic necrosis virus (PMNV), Paris virus 1 and pepper mild mottle virus. The three new viruses were tentatively named Paris potyvirus 3 (ParPV-3), Paris potyvirus 4 (ParPV-4), Paris nepovirus 1 (ParNV-1) and their complete genome sequences were determined. Sequence analyses showed ParPV-3 and ParPV-4 shared the highest amino acid (aa) sequence identities of 54.3% to each other and 53.0-57.8% to other known potyviruses. ParNV-1 had aa sequence identities of 28.8-63.7% at protease-polymerase (Pro-Pol) with other nepoviruses. Phylogenetic analyses further support that t ....

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