A recombinant spike protein subunit vaccine confers protective immunity against SARS-CoV-2 infection and transmission in hamsters
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A recombinant spike protein subunit vaccine confers protective immunity against SARS-CoV-2 infection and transmission in hamsters
sciencemag.org - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from sciencemag.org Daily Mail and Mail on Sunday newspapers.
45 Meanwhile, this protective effect of CypA was also found in cardiomyocytes.
46 Oxidative stress is believed to contribute to asthma pathogenesis, and a previous study reported that antioxidant therapy can be useful for asthma.
47 Therefore, the mechanisms of CypA on oxidative stress involved in asthma should be noted. Thus far, our understanding of the mechanisms of CypA is far from complete. The role of CypA in the functioning of asthma with regard to the mechanism of reducing the RL and the isometric tension of tracheal spiral is unknown and is the subject of further study.
Conclusion
In summary, this work has demonstrated the effects of rCypA in asthma in both OVA-challenged asthmatic rats in vivo and ACh-induced contraction tracheal spirals ex vivo. The study also identified the underlying mechanism by which rCypA may have a precise role in limiting inflammation in asthma by suppressing the secretion of Th2 cytokines and balancing the ratio of Th1/Th2 cytokines, indicating
Cellular Uptake
The cellular uptake properties of the proteins labeled with FITC were analyzed by flow cytometry. The uptake of HFtn by tumor cells was mediated by TfR1 has been proved previously. As shown in Figure 3, cellular uptake of HFtn was much higher than that of free FITC, which is probably attributed to the TfR1 receptor-mediated endocytosis. Significantly, the mean fluorescence intensity of FITC-tLyP-1-HFtn was approximately three-fold higher than FITC-HFtn, suggesting that the enhanced intracellular uptake of tLyP-1-HFtn was probably attributed to TfR1 and NRP-1 dual receptor-mediated endocytosis. In contrast, FITC-M-tLyP-1-HFtn showed similar mean fluorescence intensity as the FITC-HFtn, indicating that the mutation at the tLyP-1 peptide sequence resulted in the inactivation of the NRP-1 receptor-mediated endocytosis.