E-Mail
IMAGE: A newly developed cryo Anti-contaminator (CERES Ice Shield) consists of a cryo shutter which protects the sample from ice contamination and thereby improves preparation of the sample by the ion-beam....
view more
Credit: MPI of Molecular Physiology
In comparison to the conventional light microscopy, cryo electron tomography is a powerful tool that offers much deeper insights into the cell structure by unveiling high-resolution 3D views of biological samples such as large macromolecules. In the first step, samples are immobilized by high-speed freezing at extreme temperatures under ?150 °C. These cryogenic conditions make it possible to study samples without chemical fixation or dehydration, thereby preventing distortion or disruption of biological structures of the sample. However, the preparation and the handling of the samples are very complex, since the formation of crystalline ice can compromise the structural integrity of the specimen. Sebastian Tacke and colleagues from the MPI in Dortmund developed an innovative cryo-ET technology, which is now commercialized by the Dutch company Delmic, who specialize in offering solutions for light and electron microscopy. The new set of powerful cryo solutions is aimed to simplify and bring efficiency in the preparation phase involving cryo-ET samples.