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E-Mail IMAGE: A newly developed cryo Anti-contaminator (CERES Ice Shield) consists of a cryo shutter which protects the sample from ice contamination and thereby improves preparation of the sample by the ion-beam.. view more Credit: MPI of Molecular Physiology In comparison to the conventional light microscopy, cryo electron tomography is a powerful tool that offers much deeper insights into the cell structure by unveiling high-resolution 3D views of biological samples such as large macromolecules. In the first step, samples are immobilized by high-speed freezing at extreme temperatures under ?150 °C. These cryogenic conditions make it possible to study samples without chemical fixation or dehydration, thereby preventing distortion or disruption of biological structures of the sample. However, the preparation and the handling of the samples are very complex, since the formation of crystalline ice can compromise the structural integrity of the specimen. ....
New product to be launched in March simplifies electron cryotomography Delmic Cryo B.V. and Max-Planck-Innovation GmbH have signed a licensing agreement for production and distribution of the Cryo Workflow Tools invented and developed at the Max Planck Institute (MPI) for Molecular Physiology. The Cryo Workflow Tools are aimed at reducing ice contamination, which is a major obstacle in the cryo electron tomography (cryo-ET) workflow. The new product with the name “Delmic CERES Ice Defence System” will be launched in March 2021. It will help to boost throughput and to simplify the acquisition of new knowledge in life sciences. Electron cryotomography creates high-resolution 3D views of samples. The imaging technique is widely used in life sciences to examine biological macromolecules and cells. Due to its higher resolution, it offers much deeper insights into the cell structure than conventional light microscopy. To do this, the samples are immobilized in non-crystallin ....