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Chitosan-coated-PLGA nanoparticles enhance the antitumor

Reporting entrapment of antimetastatic Signal Transducer and Activator of Transcription 3 dimerization blocker, Stattic (S) into a chitosan-coated nanocarrier ....

Grand Island , United States , United Kingdom , Baden Wüberg , San Diego , South Korea , Malvern Zetasizer Nano , Carl Zeiss Libra , Merck Darmstadt , Malvern Instruments Ltd , Drug Administration , Committee Of The University Malaya , Nikon Corp , Roswell Park Memorial Institute , Department Of Chemistry , University Of Malaya , Beckman Coulter , Graphpad Software , Signal Transducer , Medchem Express , Monmouth Junction , Entrapment Efficiency , Releasing Profile , Phosphate Buffer Saline , Animal Experimental Unit , Medicine Institutional Animal Care ,

Difference Between Oris Cell Migration Assay and Scratch Assay

ECM Integrity Assay
In order to test the integrity of each assay’s ECM, 100 µg/mL of Collagen Type I – FITC conjugate (Sigma-Aldrich) was coated onto wells of an Oris™ assay plate (without stoppers) and a Costar
® plate. These plates were then incubated overnight at 37 °C / 5% CO
2.
After incubation, stoppers were inserted into the Oris™ assay plate while sterile PBS was added to both assay plates’ wells. At 1 hour, 24 hour, and 48 hour intervals post-insertion, stoppers were removed before fluorescence images were acquired using a Zeiss Axiovert inverted microscope.
Scratches were made in the Costar
® plate at each time point. Images were acquired using the same settings employed for the Oris™ assay plate. ....

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Difference Between Oris Cell Migration Assay and Scratch Assay

Difference Between Oris Cell Migration Assay and Scratch Assay
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