CircTMCO3 promotes gastric cancer progression
dovepress.com - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from dovepress.com Daily Mail and Mail on Sunday newspapers.
Looking Back at NIST Patents
miragenews.com - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from miragenews.com Daily Mail and Mail on Sunday newspapers.
Cell Migration and Invasion Assays by Platypus Technologies
In order to ensure consistent cell-free regions for each experiment, Cell Migration and Invasion Assays by Platypus Technologies utilize exclusion zone technology. The Oris™ Cell Migration and Invasion Assays allow the reproducible and accurate quantification of cell movement.
Cell Migration Assays
No artificial membranes
Industry-standard 96- or 384-well formats
Image Credit: Platypus Technologies, LLC
In order to form a central cell-free detection zone in the center of every well of a 96-well plate, Oris™ Cell Migration assays utilize physical “stopper” barriers.
Utilize Oris™ for maximal flexibility in assay design.
Utilize Oris™ with the Oris™ Detection Mask for analysis on plate readers that lack virtual masking.
ECM Integrity Assay
In order to test the integrity of each assay’s ECM, 100 µg/mL of Collagen Type I – FITC conjugate (Sigma-Aldrich) was coated onto wells of an Oris™ assay plate (without stoppers) and a Costar
® plate. These plates were then incubated overnight at 37 °C / 5% CO
2.
After incubation, stoppers were inserted into the Oris™ assay plate while sterile PBS was added to both assay plates’ wells. At 1 hour, 24 hour, and 48 hour intervals post-insertion, stoppers were removed before fluorescence images were acquired using a Zeiss Axiovert inverted microscope.
Scratches were made in the Costar
® plate at each time point. Images were acquired using the same settings employed for the Oris™ assay plate.