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ADAR1 inhibits HBV in PA treated HepG2.2.15

Investigating the effect and mechanism of lipotoxicity on HBV DNA replication. ....

United States , United Kingdom , Eteläuomen Läi , Baden Wüberg , Lipofectamine Rnaimax Invitrogen , Genomeditech Co Ltd , Soil Red , Thermo Fisher , Pcr Kit Accurate Biotechnology Hunan Co Ltd , Zhejiang University Medical Center , Cell Signaling Technology , Graphpad Software , Abbott Laboratories , Ge Healthcare , Adenosine Deaminases Acting , Shandong Provincial Hospital , Cell Counting , Invitrogen United States , Reverse Transcription , Quantitative Real , Polymerase Chain Reaction , Nanodrop Thermo , Premix Pro Taq , Accurate Biotechnology , Roche Diagnostics , Amersham Imager ,

A cancer ubiquitome landscape identifies metabolic reprogramming as target of Parkin tumor suppression

A cancer ubiquitome landscape identifies metabolic reprogramming as target of Parkin tumor suppression
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United States , Lipofectamine Rnai , Lipofectamine Rnaimax Invitrogen , Electron Microscopy Sciences , Mattek Corporation , Gg Kit Cell Signaling Technology , Jackson Laboratory , Wistar Institute , Like Parkin , American Type Culture Collection , Protease Inhibitor Cocktail , Phosphatase Inhibitor Cocktail , Thermo Fisher Scientific , Mitotracker Green , Ubiquitin Remnant Motif , Cell Signaling Technology , Pierce High , Avanti Polar Lipids , Thermo Fisher Scientificq Exactive , Vanquish Horizon , Laser Confocal Microscope , Focal Adhesion Analysis Server , Tokai Hit , Manual Tracking , Migration Tool , Growth Factor Reduced Matrigel ,

Dysregulation of Zinc Finger Protein 395 Contributes to the Pathogenes

All data are presented as the Mean ± SD. The Student’s
t-test or the Pearson’s Chi-square test/Fisher’s exact test was utilized to analyze difference between groups with continuous variables or categorical variables, respectively. The Kaplan-Meier method and the Log rank test were applied for overall survival with chondrosarcoma patients. Univariate and multivariate analyses of clinicopathological factors, ZNF395 level and overall survival were performed using Cox regression model. Statistical analyses were performed using SPSS (SPSS, Chicago, IL, USA) and
P ....

Grand Island , United States , Pearson Chi Square , Lipofectamine Rnaimax Invitrogen , Bright Panel , Becton Dickinson , Cell Quest Software , Left Panel , Chondrosarcoma Cell , American Type Culture Collection , Applied Biosystems , Immobilon Western Chemiluminescent , Gentian Violet , Cell Apoptosis , Assay Kit , Protein Expression , Clinicopathological Factors , Independent Prognostic , Cox Regression Analysis , Prognostic Factors , Overall Survival , Chondrosarcoma Growth , மாபெரும் தீவு , ஒன்றுபட்டது மாநிலங்களில் , பியர்சன் சி சதுரம் , சரி குழு ,

Pooled CRISPR screening identifies m6A as a positive regulator of macrophage activation

Cd86), were down-regulated in
Mettl3-deficient cells (Fig. 1G), suggesting that METTL3 has a critical function in controlling the innate immune response of Raw 264.7 macrophages.
To further confirm the biological role of the m
6A modification in macrophages,
Mettl3 conditional KO (CKO) mice were generated by crossing
Mettl3
flox/flox mice with mice expressing Cre recombinase under the control of lysozyme 2 promoter (
Lyzm-Cre). We have documented the loss of both the METTL3 protein and the overall m
6A modification in bone marrow–derived macrophages (BMDMs) from
Mettl3
flox/flox;
Lyzm-Cre mice (fig. S1I). No differences in the frequency of major immune cell populations were observed between ....

United States , Agilent Bioanalyzer , Amersham Hybond , Merck Millipore , Hua Bing Li , Lipofectamine Rnaimax Invitrogen , Shanghai Jiao Tong University School Of Medicine , National Center , National Natural Science Foundation Of China , Q Zou Shanghai Institute Of Immunology , Zai Lab Ltd , Library Prep Human Mouse Rat Illumina , Yale University School Of Medicine , Technology Committee , Ge Healthcare , National Key Rd Program Of China , R Af Department Of Immunobiology , Howard Hughes Medical Institute , Bar Harbor , Animal Care , House Committees , Shanghai Jiao Tong University School , Shanghai Institute , Shanghai Jiao Tong University , Biotechnology Information , Gene Expression Omnibus ,

[Full text] microR-4449 Promotes Colorectal Cancer Cell Proliferation via Regulati

6 MiRNAs are critical components of competing endogenous RNA network in cells.
7 Via binding to complementary sites on mRNA 3ʹUTR, miRNAs promote mRNA degradation or inhibit mRNA translation to repress gene expression.
8 Dysregulation of miRNAs results in abnormal expression of target genes and is associated with cancer development.
9 Hundreds of cancer-associated miRNAs have been reported in colorectal cancer.
10,11 For example, miR-193a-3p, which is downregulated in BRAF-mutated colorectal cancers, inhibits colorectal cancer cell proliferation.
12 MiR-4449 is highly expressed in serum from patients with multiple myeloma compared with samples from healthy volunteers.
13 However, the precise function of miR-4449 in colorectal cancer is not known. ....

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