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Enhanced glycolysis resistance against photon irradiation

Investigating the effects of enhanced glycolysis on the response of human glioma cell lines to carbon ion irradiation compared to the response to X-rays. ....

United States , New York , Anant Bhatt , Amersham Hybond , Amersham Biosciences , Cytoflexs Beckman Coulter , Biorad Laboratories Inc , Oxford Optronix Ltd , Graphpad Software , Life Sciences , Santa Cruz Biotechnology Inc , Absin Bioscience Inc , Plain Language , Santa Cruz , Beyotime Biotechnology , Absin Bioscience , Cayman Chemical , Rad Laboratories Inc , American Type Culture Collection , Ion Irradiation , Thermo Scientific , Chemidoc Image , Growth Inhibition , Cell Cycle Distribution , Beckman Coulter , Graphpad Prism ,

Pooled CRISPR screening identifies m6A as a positive regulator of macrophage activation

Cd86), were down-regulated in
Mettl3-deficient cells (Fig. 1G), suggesting that METTL3 has a critical function in controlling the innate immune response of Raw 264.7 macrophages.
To further confirm the biological role of the m
6A modification in macrophages,
Mettl3 conditional KO (CKO) mice were generated by crossing
Mettl3
flox/flox mice with mice expressing Cre recombinase under the control of lysozyme 2 promoter (
Lyzm-Cre). We have documented the loss of both the METTL3 protein and the overall m
6A modification in bone marrow–derived macrophages (BMDMs) from
Mettl3
flox/flox;
Lyzm-Cre mice (fig. S1I). No differences in the frequency of major immune cell populations were observed between ....

United States , Agilent Bioanalyzer , Amersham Hybond , Merck Millipore , Hua Bing Li , Lipofectamine Rnaimax Invitrogen , Shanghai Jiao Tong University School Of Medicine , National Center , National Natural Science Foundation Of China , Q Zou Shanghai Institute Of Immunology , Zai Lab Ltd , Library Prep Human Mouse Rat Illumina , Yale University School Of Medicine , Technology Committee , Ge Healthcare , National Key Rd Program Of China , R Af Department Of Immunobiology , Howard Hughes Medical Institute , Bar Harbor , Animal Care , House Committees , Shanghai Jiao Tong University School , Shanghai Institute , Shanghai Jiao Tong University , Biotechnology Information , Gene Expression Omnibus ,