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Rapid Technological Development and Government Initiatives

Dublin, Oct. 23, 2023 (GLOBE NEWSWIRE) The ....

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Role of LINC00504 in proliferation and invasion of BC.

http://www.mircode.org/) were used to identify the potential binding sequences of miR-140-5p and LINC00504. SKBR3 and MDA-MB-231 cells were seeded in 24-well plates. The cells were cotransfected with miR-140-5p or microRNA NC and luciferase reporter constructs containing WT-LIC00504 or Mut-LINC00504, as previously described.
13 The luciferase activity was detected using a Dual Luciferase Reporter Gene Assay Kit (RG027, Beyotime Institute of Biotechnology, Shanghai, China), according to the manufacturer’s protocol. Mutations in the putative binding sites of LINC00504 were made using a Quick-change site-directed mutagenesis kit (200518, Agilent Technologies, Santa Clara, USA). The primers were as follows: LINC00504, Forward primer: 5ʹ-GTGACTCGAGCTTGCCTCTGCCATGT-3ʹ, Reverse primer: 5ʹ-GTGAGCGGCCGCTTTCAGAGTGAAACAATACTT-3ʹ; Mut-LINC00504, Forward primer: 5`-GGATTACAGGCGTGAGGGTGAC-3`; and Reverse primer: 5`-GTCACCCTCACGCCTGTAATCC-3`. ....

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[Full text] Long Non-Coding RNA HOXA11-AS Modulates Proliferation, Apoptosis, Meta

25 Nevertheless, the reports of ITGA9 in melanoma are few.
The objective of our present study was to explore the role of HOXA11-AS in melanoma and the regulatory mechanism among HOXA11-AS, miR-152-3p and ITGA9 in cellular processes, intending to promote the understanding of the molecular pathogenesis in melanoma.
Materials and Methods
Ethic Statement
For clinical human samples, our study was performed after obtaining ratification from the Ethics Committee of The First Affiliated Hospital of Zhengzhou University. The research has been carried out in accordance with the World Medical Association Declaration of Helsinki, and that all subjects provided written informed consent. The animal experiment in this research was authorized by the Experimental Animal Ethics Committee of The First Affiliated Hospital of Zhengzhou University. Animal studies were performed in compliance with the ARRIVE guidelines and the Basel Declaration. All animals received humane care according ....

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