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Transcriptional landscapes of floral meristems in barley


Abstract
Organ development in plants predominantly occurs postembryonically through combinatorial activity of meristems; therefore, meristem and organ fate are intimately connected. Inflorescence morphogenesis in grasses (Poaceae) is complex and relies on a specialized floral meristem, called spikelet meristem, that gives rise to all other floral organs and ultimately the grain. The fate of the spikelet determines reproductive success and contributes toward yield-related traits in cereal crops. Here, we examined the transcriptional landscapes of floral meristems in the temperate crop barley (
Hordeum vulgare L.) using RNA-seq of laser capture microdissected tissues from immature, developing floral structures. Our unbiased, high-resolution approach revealed fundamental regulatory networks, previously unknown pathways, and key regulators of barley floral fate and will equally be indispensable for comparative transcriptional studies of grass meristems. ....

Nordrhein Westfalen , Baden Wüberg , Neu Isenburg , Zeiss Axioskop , U Lundqvist Nordgen , Zeiss Stemi , Program Of The German Research Foundation , European Research Council , Ministry Of Education , Cell Cut , Isolation Caps , Illumina Truseq , European Nucleotide Archive , Dynamic Hybrid Tree , Roche Diagnostics , Axio Vision , Creative Commons Attribution Noncommercial , Federal Ministry , German Research Foundation , Ontario Genomics , னோற்ர்ேன் வெஸ்ட்ஃபாலன் , ஐரோப்பிய ஆராய்ச்சி சபை , அமைச்சகம் ஆஃப் கல்வி , ரோச் பரிசோதனை , கூட்டாட்சியின் அமைச்சகம் , ஜெர்மன் ஆராய்ச்சி அடித்தளம் ,