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Adding α,α-disubstituted and β-linked monomers to the genetic code of an organism

The genetic code of living cells has been reprogrammed to enable the site-specific incorporation of hundreds of non-canonical amino acids into proteins, and the encoded synthesis of non-canonical polymers and macrocyclic peptides and depsipeptides1–3. Current methods for engineering orthogonal aminoacyl-tRNA synthetases to acylate new monomers, as required for the expansion and reprogramming of the genetic code, rely on translational readouts and therefore require the monomers to be ribosomal substrates4–6. Orthogonal synthetases cannot be evolved to acylate orthogonal tRNAs with non-canonical monomers (ncMs) that are poor ribosomal substrates, and ribosomes cannot be evolved to polymerize ncMs that cannot be acylated onto orthogonal tRNAs—this co-dependence creates an evolutionary deadlock that has essentially restricted the scope of translation in living cells to α-l-amino acids and closely related hydroxy acids. Here we break this deadlock by developing tRNA d ....

El Yacoubi , Melo Czekster , Acta Crystallogr , Acids Res , Protein Sci , Peptide Sci , Illumina Paired End ,

Frontiers | Microbial Communities on Plastic Polymers in the Mediterranean Sea

Frontiers | Microbial Communities on Plastic Polymers in the Mediterranean Sea
frontiersin.org - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from frontiersin.org Daily Mail and Mail on Sunday newspapers.

Gulf Of Mannar , Oceans General , United States , Himachal Pradesh , United Kingdom , Gulf Of Mexico , Minas Gerais , Caribbean Sea , Belize General , New Zealand , Mediterranean Sea , New Zealand General , Rio Grande Do Norte , Germany General , North Sea , Atlantic Zettler , Pacific Bryant , Atlantic Ocean Zettler , Sea Tu , Cutadapt Martin , Sea Cozar , Sea Oberbeckmann , Cruz Viggi , Sea Kirstein , Van Franeker , Herrero Acero ,