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Tokyo Institute Of Technology: Unraveling the Molecular Basis of Dmc1 Filament Assembly in Homologous Recombination

Hop2-Mnd1 and Swi5-Sfr1 stimulate Dmc1-driven homologous recombination by acting on different steps during filament assembly, reveal Tokyo Tech researchers in collaboration with National Taiwan University. Using two types of single-molecule experimen ....

National Taiwan University , T Ai Pei , Hideo Tsubouchi , Owen Li , Nucleic Acids Research , Tokyo Institute Of Technology Tech , Tokyo Tech , National Taiwan , Assistant Professor Hideo Tsubouchi , Tokyo Institute ,

Decoding Dmc1 Filament Assembly in Homologous Recombination

Decoding Dmc1 Filament Assembly in Homologous Recombination
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National Taiwan University , T Ai Pei , Owen Li , Hideo Tsubouchi , Tokyo Institute Of Technology Tech , Nucleic Acids Research , Assistant Professor Hideo Tsubouchi , Tokyo Institute , Tokyo Tech ,

Unraveling the molecular basis of Dmc1 filament assembly in homologous recombination

Unraveling the molecular basis of Dmc1 filament assembly in homologous recombination
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National Taiwan University , T Ai Pei , Hideo Tsubouchi , Owen Li , Wei Lee , Nucleic Acids Research , Tokyo Institute Of Technology Tech , Tokyo Tech , Assistant Professor Hideo Tsubouchi , Tokyo Institute , Nucleic Acids ,

Sharing shears: Conserved protein segment activates molecular DNA scissors for DNA repair


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IMAGE: CtIP/Ctp1 stimulates endonuclease activity of the MRN complex, which not only polishes dirty ends of DNA breaks, but promotes accurate repair of DNA breaks through the homologous recombination mechanism.
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Credit: PNAS
Scientists at Tokyo Institute of Technology (Tokyo Tech) have uncovered mechanisms underlying the activation of the MRN complex the cell s DNA scissors. Using purified yeast proteins, they demonstrated that phosphorylation of Ctp1, a homolog of a tumor-suppressor protein, plays a key role in activating MRN complex s DNA clipping activity. Intriguingly, a short segment of yeast Ctp1 or its human counterpart could stimulate endonuclease activity of their respective MRN complexes, suggesting its conserved function across species. ....

Hiroshi Iwasaki , Hideo Tsubouchi , Iwasaki Laboratory , Tokyo Institute Of Technology , Tokyo Institute , Tokyo Tech , Assistant Professor Hideo Tsubouchi , Professor Hiroshi Iwasaki , C Terminal Ctp , Uncovering How Different Proteins Cooperate , Cell Biology , ஹிரோஷி இவாசகி , இவாசகி ஆய்வகம் , டோக்கியோ நிறுவனம் ஆஃப் தொழில்நுட்பம் , டோக்கியோ நிறுவனம் , டோக்கியோ தொழில்நுட்பம் , ப்ரொஃபெஸர் ஹிரோஷி இவாசகி , செல் உயிரியல் ,