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A comprehensive investigation on cells and colony motion offers new insight into the proliferative and epithelial regenerative capacities of human primary oral keratinocyte cultures with implications for quality control of engineered cells used in regenerative medicine. ....
Cells/colony motion of oral keratinocytes was non-invasively and quantitatively determined by optical flow algorithm. As per the distinct cell growth kinetics, modified optic flow algorithm was applied with fewer full-screening imaging analyses & cell segmentations, which confirmed the association of proliferative capacity & epithelial regenerative capacity with mean motion speed (MMS). The index of MMS was identified to detect the substandard population, prior to human clinical use, which may be applied to other cell cultures. ....