Evaluating the potential influence of puerarin on the proliferation and osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (BMSCs).
Frontiers | Nuclear Receptor PXR Confers Irradiation Resistance by Promoting DNA Damage Response Through Stabilization of ATF3 frontiersin.org - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from frontiersin.org Daily Mail and Mail on Sunday newspapers.
Luciferase Reporter Assays
The circ-000543 wild sequence, ZNF268-3′UTR wild sequence and their mutations with or without miR-567 binding sites were synthesized and then subcloned into luciferase reporter vectors psiCHECK-2, which was bought from Promega (Madison, WI, USA). HEK293T cells were implanted in 24-well plates at the density of 3×10
4 cells/well in triplicate. Then, miR-567 or NC mimic were cotransfected with corresponding psiCHECK-2 plasmids. After co-transfection for 48 h, dual-luciferase reporter assay system that provided by Promega was proceeded to conduct the luciferase reporter assays according to the manufacturer’s instructions. Relative luciferase activity was subsequently normalized to the Renilla luciferase internal control.
miR-593-3p Promotes Proliferation in Prostate Cancer dovepress.com - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from dovepress.com Daily Mail and Mail on Sunday newspapers.