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A spatially resolved timeline of the human maternal–fetal interface

Beginning in the first trimester, fetally derived extravillous trophoblasts (EVTs) invade the uterus and remodel its spiral arteries, transforming them into large, dilated blood vessels. Several mechanisms have been proposed to explain how EVTs coordinate with the maternal decidua to promote a tissue microenvironment conducive to spiral artery remodelling (SAR)1–3. However, it remains a matter of debate regarding which immune and stromal cells participate in these interactions and how this evolves with respect to gestational age. Here we used a multiomics approach, combining the strengths of spatial proteomics and transcriptomics, to construct a spatiotemporal atlas of the human maternal–fetal interface in the first half of pregnancy. We used multiplexed ion beam imaging by time-of-flight and a 37-plex antibody panel to analyse around 500,000 cells and 588 arteries within intact decidua from 66 individuals between 6 and 20 weeks of gestation, integrating this dataset ....

Leica Biosystems , Dako Agilent , Nanostring Geomx , Flowsom Bioconductor Flow , Perivascular Evts , Electron Microscopy Sciences , Lab Vision Pt Module Thermo Fisher Scientific , Sigma Aldrich , Institutional Review Board At Stanford University , Fisher Scientific , Stanford University , Beckman Coulter , Thermo Fisher , Supplementary Table , Stanford Tissue Microarray Database , Supplementary Information , Candor Bioscience , Thermo Fisher Scientific , Flab Vision , Cell Marque , Biotin Blocking , Deepcell Label , Vector Laboratories , Vector Blue , Nanozoomer Digital Pathology Scanner , Extended Data ,

Mutation markers: Improved diagnostics for neuropathologies

Explore the value of mutation markers for improved cancer diagnosis, classification, and prediction of prognosis. ....

United States , Qin Su , Brent Heller , Editorial Article , Cell Marque , Tissue Diagnostics , Cell Marque Tissue Diagnostics , The Value Of Mutation Markers For Personalized Medicine In Neuropathology Selectscience , Tissue Diagnostics ,

Mutation markers: Improved diagnostics for neuropathologies

Explore the value of mutation markers for improved cancer diagnosis, classification, and prediction of prognosis. ....

United States , Qin Su , Brent Heller , Editorial Article , Cell Marque , Tissue Diagnostics , Cell Marque Tissue Diagnostics , The Value Of Mutation Markers For Personalized Medicine In Neuropathology Selectscience , Tissue Diagnostics ,

Cureus | Expression of c-Myc, Bcl2, Bcl6, and Cyclin D1 in High-Grade B-Cell Lymphoma

Background

High-grade B-cell lymphomas (HGBLs), with c-Myc, Bcl2, and/or Bcl6 rearrangement, are aggressive neoplasms with poor clinical outcomes. Cyclin D1 is a proto-oncogene that is generally expressed by mantle cell lymphoma, its variants, and many other neoplasms.

Aim

The aim of this study is to investigate the expression and frequency of cyclin D1 in HGBL and its association with double expressor and triple expressor lymphomas. A few pieces of research have been reported on the expression of cyclin D1 in triple-hit lymphoma, renaming it quadruple-hit lymphoma. In the present study, we also used immunohistochemical (IHC) stains to look at the expression of cyclin D1 in double expressor and triple expressor.

Methodology

This is a cross-sectional descriptive study involving 60 cases, including both double and triple expressors, diagnosed by using hematoxylin and eosin (H&E) staining with subsequent IHC stains. We analyzed the expression of ....

United States , Nexus Software Kirtland , World Health Organization , Statistical Package Of Social Sciences , Chughtai Institute Of Pathology , Yc Nuclear , Chughtai Institute , Pathology Institutional Review Board , Nexus Software , Rabit Monoclonal , Cell Marque , Social Sciences ,

Postmortem Assessment of Olfactory Tissue Degeneration and Microvasculopathy in Patients With COVID-19

This cohort study evaluates the association of COVID-19 infection with olfactory bulb by performing an ultrastructural and histopathological analysis of patient ....

United States , Johns Hopkins University , National Institutes Of Health , University Of Maryland Medical Center , Examiner Or Orlando Health , University Of Maryland Baltimore , Drug Administration , Us Centers For Disease , Johns Hopkins University School Of Medicine , Thermo Fisher , Clinical Laboratory Improvement Amendments , Department Of Pathology , Disease Control , Observational Studies , Developing Drugs , Biological Products , Maryland Medical Center , Maryland Office , Chief Medical Examiner , Orlando Health , Microvascular Pathology , Density Analyses , Bio Rad Laboratories , Antigen Retriever Citrate Buffer , Alexa Fluor , Carl Zeiss ,