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Methods and Data
Tissue Samples
A total of 66 cases of OS patients treated in Yantai Affiliated Hospital of Binzhou Medical University from January 2010 to January 2014 were collected. Cancer tissues and paracancerous tissues of the patients were collected during the operation, transported with liquid nitrogen and sent to the laboratory for testing. The tissues were stored at −80°C. Peripheral blood (3 mL) was collected from patients preoperatively, and peripheral blood (3 mL) was also collected from 66 cases of healthy subjects simultaneously. The obtained peripheral blood was placed in the test tube. The samples were incubated at 37°C for 1 hour and centrifuged at 1500 x ....

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