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Lipid-Based Nanoparticles: Manufacturing and Characterization


In drug development many applications are found for lipid-based nanoparticles (Lb-NPs) as successful transporters
for poorly water-soluble drugs and oligonucleotides in gene therapy. 
Major advantages of lipids are their
high biocompatibility and biodegradability. In addition, advanced formulations using Lb-NP encapsulation of active ingredients can strongly contribute to targeted drug delivery in the body as well as stability and storage of the formulation.
The most important current medical applications include
cancer therapeutics and some of the current
COVID-19 vaccines.  
Development and manufacturing of Lb-NP products often involves complex processes that need to be thoroughly understood and controlled to ensure optimum and constant quality of the medicine for patients. Therefore, ....

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Using Dynamic Light Scattering (DLS) to Analyze Virus Particles


Using Dynamic Light Scattering (DLS) to Analyze Virus Particles
Conventional visual techniques of detecting virus particles in solution provide a glimpse of an extremely small sampling volume, while the method of particle analysis employing dynamic light scattering generates an ensemble
average of particles inside the solutions.
Virus particles incubated in the laboratory pose an issue, as they need to be grown inside cells in a media containing albumin and additional small proteins, like those inside Fetal Bovine Serum (FBS) or Minimum Essential Media (MEM) solutions.
When virus particles are emitted from the cells, the cell pieces are big and can be divided by centrifugation, however the lesser proteins of the media cannot be eliminated. Cautious choice of the distribution parameters from a dynamic light scattering experiment permits clear observation of the size distributions of virus particles in the presence of significantly smaller proteins t ....

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