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Cell Culture Basics: Equipment, Fundamentals and Protocols


- Cell counting
What is cell culture?
Cell culture refers to the removal of cells from an animal or plant and subsequent cultivation in an artificial environment for scientific research. The first cell culture techniques were developed over 100 years ago and since then have contributed to tremendous breakthroughs in science. Today, it is a fundamental tool used in laboratories around the world for studying the normal physiology and biochemistry of cells, mechanisms underlying disease and effects of drugs and toxic compounds. It is also used in drug screening and development and large-scale manufacturing of biological compounds, such as vaccines and therapeutic proteins. ....

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Diagnosis and precise nanocarrier-bowel disease therapy


82 and lipid nanoparticles
74 have been developed and used in cancer imaging and diagnosis. During traditional clinical CT imaging, iodinated small molecules and barium sulfate suspensions are usually used as contrast agents,
121,122 but they are not ideal for imaging IBD due to their nonspecificity. Furthermore, when administered orally, these suspensions are nonspecifically distributed throughout the GIT, thus interfering with diagnosis and leading to accumulative radiation exposure in IBD patients.
123 Therefore, the CT contrast agent must not only be stable in the GIT microenvironment but also must be specifically enriched in the diseased parts of the intestine to better detect disease.
Based on the microenvironmental characteristics of the GIT and the design strategy of orally administered DDSs for IBD therapy, the application of nanoparticles may create ideas for the CT imaging in IBD. Naha et al designed and synthesized nanocontrast agents that can be used i ....

Saudi Arabia , Sri Lanka , Nano Lett , Panchagnular Peroral , Liuf Immunomodulation , Acta Biomater , Jons Biotinylated , Vllasaliud Ascorbyl , Daneses Biosimilar , Carbohydr Polym , Pan Stanford , Gastroenterol Hepatol , Jpharm Biopharm , Traversog Nanoparticulate , Macromol Biosci , Scandj Gastroenterol , National Natural Science Foundation Of China , Springer International Publishing , National Natural Science Foundation , Innovative Research Team , Rev Gastroenterol , Inflammatory Bowel , Shiht Y , Opin Drug , Mater Chem , Dufresnem H ,