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Diagnosis of Legionnaires' Disease

Metagenomics is a promising approach that facilitated the diagnosis of Legionnaires’ disease ....

United States , Santa Clara , Agilent Technologies , Library Prep Kit , Tissue Kit , Thermo Fisher , Prep Kit , About Legionnaire ,

Phylogenetic evidence for a clade of tick-associated trypanosomes | Parasites & Vectors

Trypanosomes are protozoan parasites of vertebrates that are of medical and veterinary concern. A variety of blood-feeding invertebrates have been identified as vectors, but the role of ticks in trypanosome transmission remains unclear. In this study, we undertook extensive molecular screening for the presence and genetic diversity of trypanosomes in field ticks. Examination of 1089 specimens belonging to 28 tick species from Europe and South America led to the identification of two new trypanosome strains. The prevalence may be as high as 4% in tick species such as the castor bean tick Ixodes ricinus, but we found no evidence of transovarial transmission. Further phylogenetic analyses based on 18S rRNA, EF1-α, hsp60 and hsp85 gene sequences revealed that different tick species, originating from different continents, often harbour phylogenetically related trypanosome strains and species. Most tick-associated trypanosomes cluster in a monophyletic clade, the Trypanosoma pestanai cl ....

French Guiana , Tissue Kit , Thermo Scientific , Roche Diagnostics , Eurofins Genomics , Chromas Lite ,

Establish an allele-specific real-time PCR for Leishmania species identification | Infectious Diseases of Poverty

Leishmaniasis is a serious neglected tropical disease that may lead to life-threatening outcome, which species are closely related to clinical diagnosis and patient management. The current Leishmania species determination method is not appropriate for clinical application. New Leishmania species identification tool is needed using clinical samples directly without isolation and cultivation of parasites. A probe-based allele-specific real-time PCR assay was established for Leishmania species identification between Leishmania donovani and L. infantum for visceral leishmaniasis (VL) and among L. major, L. tropica and L. donovani/L. infantum for cutaneous leishmaniasis (CL), targeting hypoxanthine-guanine phosphoribosyl transferase (HGPRT) and spermidine synthase (SPDSYN) gene with their species-specific single nucleotide polymorphisms (SNPs). The limit of detection of this assay was evaluated based on 8 repeated tests with intra-assay standard deviation < 0.5 and inter- ....

United States , New Zealand , Sangon Biotech Co Ltd , Capital Medical University , Beijing Friendship Hospital , Universidad Nacional De Salta Argentina , Ibis Biosciences , Applied Biosystems Roche , Basic Local Alignment Search Tool , Tissue Kit , Promega Gotaq Probe , Applied Biosystems , Mega Limited , Neighbor Joining ,