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Phylogenetic evidence for a clade of tick-associated trypanosomes | Parasites & Vectors

Trypanosomes are protozoan parasites of vertebrates that are of medical and veterinary concern. A variety of blood-feeding invertebrates have been identified as vectors, but the role of ticks in trypanosome transmission remains unclear. In this study, we undertook extensive molecular screening for the presence and genetic diversity of trypanosomes in field ticks. Examination of 1089 specimens belonging to 28 tick species from Europe and South America led to the identification of two new trypanosome strains. The prevalence may be as high as 4% in tick species such as the castor bean tick Ixodes ricinus, but we found no evidence of transovarial transmission. Further phylogenetic analyses based on 18S rRNA, EF1-α, hsp60 and hsp85 gene sequences revealed that different tick species, originating from different continents, often harbour phylogenetically related trypanosome strains and species. Most tick-associated trypanosomes cluster in a monophyletic clade, the Trypanosoma pestanai cl ....

French Guiana , Tissue Kit , Thermo Scientific , Roche Diagnostics , Eurofins Genomics , Chromas Lite ,

Virulent Newcastle Disease Viruses Circulating in Ethiopia


↓ cleavage residues indicate that the NDV isolates correspond to mesogenic/velogenic pathotypes.
Figure 2 Deduced amino acid sequence alignment of F gene sequences of NDV strains isolated from Ethiopia. Names of strains are shown on the left-hand side of the figure. Identification with the reference La Sota (genotype II) vaccine strains is indicated by dots. They indicate the cleavage site of the deduced aa sequence of the F gene. X indicates an ambiguous aa sequence.
Abbreviations: Eth, Ethiopia; Chk, chicken.
Genetic Distance
The Ethiopian NDV isolates have a within-group genetic distance of 10.16% and a between-group genetic distance of 25.1% (Table 2). These isolates are grouped for ease of presentation as published by authors: group1 (sequences characterized in this study from the central part of the country), group 2 from the central part of Ethiopia, ....

Lohmann Animal Health , Office International Des Epizooties , National Veterinary Institute , Technelysium Pty Ltd , National Veterinary , Hemagglutination Inhibition , Pathogenicity Index , Diagnostic Tests , Terrestrial Animals , Gel Extraction Kit , Cycle Sequencing Kit , Applied Biosystems , Sigma Spin Post Reaction Clean Up Columns , Sanger Sequencer , Chromas Lite , Molecular Evolutionary Genetics Analysis , Minimum Evolution Method , Amino Acid Substitutions , Fusion Proteins , Standard Error , Genetic Distance Within , Two Groups , Mean Genetic Distance , Std Error Shown Above , Diagonal Line , Between Ethiopian ,