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Overexpression of a novel gene (Pt2015) endows the commercial diatom Phaeodactylum tricornutum high lipid content and grazing resistance | Biotechnology for Biofuels and Bioproducts

The marine diatom Phaeodactylum tricornutum is a commercially viable species due to its bioactive substances and lipid productivity. Increasing attention has been paid to the isolation or genetic modification of species or strains with a rapid growth rate and large quantities of lipids. Furthermore, contamination of microzooplankton has been one of the major constraints in P. tricornutum large-scale cultivation, which adversely affects growth and greatly impedes the course of biomass production industrialization. Here, based on our previous transcriptomics of P. tricornutum, we found a novel gene (ID: 7202015, hereafter called Pt2015) which affects morphotype of P. tricornutum. Pt2015 protein is located in the plastid, which is highly homologous to part of the sequences of exosome component. The morphotype of the Pt2015 knockout strain (termed 2015KO) using CRISPR/Cas9 method is fusiform, but the Pt2015 overexpression strain (termed oeT) demonstrates a majority triradiate morphotype (a ....

United States , United Kingdom , Heinz Walz , Leica Microsystems , Santa Clara , Ted Pella , National Center , Gene Denovo Biotechnology Co , Ted Pella Inc , Institute Of Hydrobiology , Chinese Academy Of Sciences , Rahmi Lale Norwegian University Of Science , Beckman Coulter , Carl Zeiss , Chinese Academy , Golden Gate , New England Biolabs , Rahmi Lale , Norwegian University , Electron Microscopy China , Thermo Fisher Scientific , French Press , San Jose , Tian Gen , Agilent Technologies , Palo Alto ,

Prosthetics for Injured Animals


Prosthetics for Injured Animals
Historically, artificial devices for wildlife have been expensive and very time-consuming to produce. 3D printing is changing that calculus by making it easier to design and build better-fitting prosthetics.
ByLaurel Neme
When caregivers at the Weltvogelpark Walsrode bird park in Germany returned to the aviary one morning, they were heartsick to discover that a secretary bird they called Söckchen, or “Little Sock,” had broken her leg inside her enclosure. They guessed that something must have scared her and made her jump, causing a severe break.
Secretary birds (Sagittarius serpentarius) need their legs. Found in Africa’s savannas, they are large, mostly terrestrial birds; their strong, sturdy legs are vital not only for walking but for capturing snakes and other prey. Often, they hunt by stomping the ground to flush out animals, then run them down and strike with their beak or feet to stun or kill them. In the wild, the loss ....

Dutch Harbor , United States , Phnom Tamao , Siem Re , New South Wales , Costa Rica , Rio De Janeiro , Estado Do Rio , Lowry Park , Bobj Galindo , Lars Thalm , Roberto Fecchio , Don Clucas , Laurel Neme , Lars Thalmann , Weltvogelpark Walsrode , Janie Veltkamp , Zealand International Antarctic Centre , Wildlife Alliance Phnom Tamao Rescue Center , Wildlife Alliance , Rescate Wildlife Rescue Center , University Of Canterbury , German Alonso , Prey Northwest , Instituto Vida Livre , Dalian Forest Zoo ,

Life and new limbs: Creative thinking, 3D printers save injured wildlife


Life and new limbs: Creative thinking, 3D printers save injured wildlife
by Laurel Neme on 5 April 2021
Prosthetics for injured animals are becoming increasingly possible and accessible thanks to 3D printing. Historically, artificial devices for wildlife have been expensive and very time-consuming to produce. 3D printing is changing that calculus by making it easier to design and build better-fitting prosthetics.
A team of dedicated caregivers with vision, creativity and persistence is often the common thread that is key to helping injured animals.
While 3D printing of animal prosthetics allows for multiple iterations that helps improve the device so that the animal can function more normally, size and materials can limit their use. ....

Dutch Harbor , United States , Phnom Tamao , Siem Re , New South Wales , Costa Rica , Rio De Janeiro , Estado Do Rio , Lowry Park , Bobj Galindo , Lars Thalm , Roberto Fecchio , Don Clucas , Lars Thalman , Lars Thalmann , Weltvogelpark Walsrode , Janie Veltkamp , Zealand International Antarctic Centre , Wildlife Alliance Phnom Tamao Rescue Center , Wildlife Alliance , Rescate Wildlife Rescue Center , University Of Canterbury , German Alonso , Prey Northwest , Fitting Beauty , Instituto Vida Livre ,