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Harvard Researchers Publish Light-Activated Spatial Transcriptomics Method

Light-Seq enables researchers to laser-spot areas of interest in tissue sections for analysis with both imaging and sequencing. ....

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Science News | Scientists Turn Up Spotlight on Cells in Tissues So RNA Could Explain Disease and Biology

Get latest articles and stories on Science at LatestLY. A new advance overcomes present limitations in spatial transcriptomics with a DNA nanotechnology-driven method called Light-Seq. Light-Seq allows researchers to geotag the full repertoire of RNA sequences with unique DNA barcodes exclusive to a few cells of interest. These target cells are selected using light under a microscope via a fast and effective photocrosslinking process, and their RNAs made available to next-generation sequencing with the help of a new DNA nanotechnology-driven technique. This entire process can then be repeated for different cell populations in the same sample. Science News | Scientists Turn Up Spotlight on Cells in Tissues So RNA Could Explain Disease and Biology. ....

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Scientists turn up spotlight on cells in tissues so RNA could explain disease and biology

A new advance overcomes present limitations in spatial transcriptomics with a DNA nanotechnology-driven method called Light-Seq. Light-Seq allows researchers to geotag the full repertoire of RNA sequences with unique DNA barcodes exclusive to a few cells of interest ....

United States , Baden Wüberg , Constance Cepko , Ninning Liu , Donald Ingber , Sinem Saka , Jocelyn Josie Kishi , Wyss Institute , Wyss Institute Molecular Robotics Initiative , Wyss Technology Development Fellow On Yin , European Molecular Biology Laboratory In Heidelberg , Group Leader , Harvard Johna Paulson School Of Engineering , Blavatnik Institute , Biologically Inspired Engineering , Harvard University , Nature Methods , Peng Yin , Core Faculty , Systems Biology , Harvard Medical School , Gemma West , Wyss Technology Development Fellow , Postdoctoral Fellow , European Molecular Biology Laboratory , Bullard Professor ,

Light-Seq technology for barcoding and deep-sequencing selected cell populations in tissues

Under the microscope, researchers often observe different cell types organizing themselves in peculiar patterns within tissues, or sometimes a rare cell type that stands out by occupying a unique position, exhibiting an unusual shape, or expressing a specific biomarker molecule. ....

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