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Abstract Trophic structures, i.e., the diets, food chain positions, and feeding relationships of species in an ecological community, are a fundamental yet understudied avenue of investigation into Australian megafauna extinction. Calcium (Ca) isotope analysis has been developed as a tool to reconstruct trophic levels in vertebrate palaeobiology and palaeoecology. A baseline of modern marsupial Ca isotope signatures in a single trophic level and dietary niche is required to successfully apply this tool in order to reconstruct Australian faunal food webs. We present Ca isotope data from dental enamel of modern Tasmanian bare-nosed wombats (Vombatus ursinus tasmaniensis) to constrain the Ca isotope composition of this species, an opportunistic grazer, and assess whether sex and environmental variables influence such composition. We compare these data to complementary strontium isotope data from the same samples as a proxy for geographical location. Elodont (ever-growing) wombat t ....