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Supported Liquid Extraction versus Liquid-Liquid Extraction: Extracting Acidic, Basic and Neutral Analytes from Plasma

Mar 11 2021 Read 153 Times
Author: James Edwards on behalf
of Porvair Sciences
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Sample preparation is a key process when running any samples on a chromatography instrument. No matter how good the instrument is, to get the best sensitivity and reproducibility sample clean-up needs to be performed. A traditional method has been to use liquid-liquid extraction (LLE) which allows transfer of analyte from an aqueous solution to an organic solution. However, LLE can have issues around the time, irreproducibility and emulsions forming.
This application note discusses the advantages of using a supported liquid extraction (SLE) over an LLE method for extracting a range of analytes (acidic, basic and neutral) from pig plasma. The Microlute™ SLE plate offers an alternative method to LLE which follows the same principles of LLE methods. It improves recovery, reproducibility and speeds up sample preparation to allow a greater t ....

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Solid Phase Extraction Microplate Selection Guide

Solid Phase Extraction Microplate Selection Guide
Porvair Sciences announce the availability of a new guide that makes selection of the optimum Solid Phase Extraction (SPE) microplate for your application both quick and easy.
SPE is a widely used method of sample preparation that concentrates and purifies analytes from a solution by means of absorption, followed by the elution of the analyte with a solvent appropriate for instrumental analysis, with techniques such as LC-MS. Choosing the correct SPE microplate for your application can mean the difference between indifferent and great results.
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The selection guide adopts an easy-to-use flow chart to enable you to quickly select the most appropriate Porvair Sciences Microlute CP SPE microplate by sample matrix (aqueous or organic), analyte charge, solubility and polarity and finally sample retention mechanism. ....

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