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Tracking cells with omnidirectional visible laser particles


Credit: by Shui-Jing Tang, Paul H. Dannenberg, Andreas C. Liapis, Nicola Martino, Yue Zhuo, Yun-Feng Xiao, and Seok-Hyun Yun
Laser particles are micrometre and nanometre lasers in the form of particles dispersible in aqueous solution, which have attracted considerable interest in the life sciences as a promising new optical probe. Laser particles emit highly bright light with extremely narrow spectral bandwidth. By transferring laser particles into live cells as shown in Figure 1, individual cells in a heterogeneous population can be tracked using each intracellular particle s specific spectral fingerprint as an optically readable barcode. However, laser particles emit directional light (Figure 2) and freely tumble inside living cells, their orientation varying randomly over time. Therefore optical readout of these labels results in lighthouse-like blinking, leading to frequent loss of cell traces. ....

Paul Dannenberg , Andreas Liapis , Yun Feng Xiao , Seok Hyun Yun , Harvard University , Harvard Medical School , Professor Seok Hyun Yun , Professor Yun Feng Xiao , Peking University , Shui Jing Tang , Harvard Medical , பால் டானன்பெர்க் , யுன் ஃபெங் கிஷியஓ , செஓக் யுங் யுன் , ஹார்வர்ட் பல்கலைக்கழகம் , ஹார்வர்ட் மருத்துவ பள்ளி , ப்ரொஃபெஸர் செஓக் யுங் யுன் , ப்ரொஃபெஸர் யுன் ஃபெங் கிஷியஓ , பேகிங் பல்கலைக்கழகம் , சுய் ஜிங் டாங் , ஹார்வர்ட் மருத்துவ ,