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SAHA Alleviates Diarrhea-Predominant IBS

SAHA Alleviates Diarrhea-Predominant Irritable Bowel Syndrome through Regulation of the p-STAT3/SERT/5-HT Signaling Pathway ....

Rocky Hill , Yedebub Biheroch Bihereseboch Na Hizboch , United States , United Kingdom , Bio Rad Chemidoc , Laboratory Animal Co Ltd , Shanghai Haoran Biotechnology Co Ltd , Shanghai Bogoo Biological Technology Co Ltd , Molecular Devices Corporation , Shanghai Yuanye Biological Technology Co Ltd , Imunbio Biotechnology Co Ltd , Laboratory Animals , Guangzhou Weijia Technology Co Ltd , Olympus Optical Co Ltd , Wuhan Boster Biological Technology , Shanghai Genepharma Co Ltd , Thermo Fisher Scientific Inc , Us National Institutes Of Health , Shanghai Fusheng Industrial Co Ltd , Animal Ethics Committee , Shuguang Hospital Affiliated , Shanghai Traditional Chinese Medical University , Animal Anesthesia Machine , Fecal Water , Animal Metabolism System , Colonic Mast ,

NLRP3 participation in inflammatory responses in labor

Hydrogen sulfide contributes to uterine quiescence through inhibition of NLRP3 inflammasome activation by suppressing the TLR4/NF-κB signalling pathway ....

United States , Eteläuomen Läi , Cell Signalling Technology , Laboratory Animal Co Ltd , Detection System Bio Rad Laboratories Inc , Elisa Rd Systems Inc , Lncbio Co , Takara Biotechnology Co Ltd , Shanghai University Of Traditional Chinese Medicine , Thermo Fisher Scientific Inc , Rt Reagent Kit Takara Biotechnology Co Ltd , Ethics Committee , Shanghai University Of Traditional Chinese Medicine No , Laboratory Animal Centre Of Putuo Hospital , Ethics Administration Office Of Pu Tuo Hospital , Ethics Administration Office , Shanghai University , Traditional Chinese Medicine , Laboratory Animal Centre , Putuo Hospital , Thermo Fisher Scientific , Sigma Aldrich , Life Technologies , Human Total , Assay Kit , Santa Cruz Biotechnology ,

Downregulation of microRNA-130a inhibits OSCC metastasis

Downregulation of microRNA-130a inhibits OSCC metastasis
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Hsa_circ_0000144 regulates miR-502-5p/ADAM9 axis

Hsa_circ_0000144 regulates miR-502-5p/ADAM9 axis
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United States , France General , United Kingdom , Franklin Lakes , Nuclear Cytoplasmic Fractionation , Ethics Committee Of Baoji Central Hospital , Laboratory Animal Co Ltd , Rna Detection Kit Genecopoeia Inc , Ethic Committee Of Baoji Central Hospital , Roswell Park Memorial Institute , Geneseed Biotech Co Ltd , Hanbio Biotechnology Co , Ethic Committee , Baoji Central , Gran Island , Goscript Reverse Transcription System , Script Reverse Transcription Kit , Genecopoeia Inc , Primer Sequences , Thermo Fisher Scientific , Epicentre Biotechnologies , Annexinv Fluorescein , Apoptosis Detection , Assay Kit , Directed Mutagenesis System , Glo Dual Luciferase ,

[Full text] KDM5C Expedites Lung Cancer Growth and Metastasis Through Epigenetic R

Reverse Transcription Quantitative PCR (RT-qPCR)
Following total RNA extraction using TRIzol reagent (Invitrogen), cDNA was synthesized using Mir-X miRNA First-Strand Synthesis Kit (Takara Holdings Inc., Kyoto, Japan) with extracted total RNA (2 μg). The miRNA expression was measured by qPCR with SYBR Advantage qPCR Premix (Takara) on CFX133a Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA). Relative expression was determined with a U6 control through the 2
−ΔΔCt method. The primers used are depicted in Table 1.
Table 1 Primer Sequences
Cell Culture, Transfection and Infection
Human lung cancer cell lines, including H522, H460, SK-MES-1, and A549, and a human bronchial epithelial cell line BEAS-2B, were from the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China). Cell lines H522, H460, and A549 were cultivated in RPMI-1640 medium (Gibco; Thermo Fisher Scientific), while SK-MES-1 cells were in m ....

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