Live Breaking News & Updates on Yeasen Biotechnology Co Ltd
Stay updated with breaking news from Yeasen biotechnology co ltd. Get real-time updates on events, politics, business, and more. Visit us for reliable news and exclusive interviews.
Exploring whether nanoparticles coated with Trimethyl chitosan (TMC) derivatives (PPTT-NPs) could promote the oral bioavailability of Panax notoginseng saponins ....
Bilirubin in the treatment of gastric ulcer & confirming that CS-BR nanoparticles are effective at alleviating acute gastric ulcer in an experimental model. ....
Reverse Transcription Quantitative PCR (RT-qPCR) Following total RNA extraction using TRIzol reagent (Invitrogen), cDNA was synthesized using Mir-X miRNA First-Strand Synthesis Kit (Takara Holdings Inc., Kyoto, Japan) with extracted total RNA (2 μg). The miRNA expression was measured by qPCR with SYBR Advantage qPCR Premix (Takara) on CFX133a Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA). Relative expression was determined with a U6 control through the 2 −ΔΔCt method. The primers used are depicted in Table 1. Table 1 Primer Sequences Cell Culture, Transfection and Infection Human lung cancer cell lines, including H522, H460, SK-MES-1, and A549, and a human bronchial epithelial cell line BEAS-2B, were from the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China). Cell lines H522, H460, and A549 were cultivated in RPMI-1640 medium (Gibco; Thermo Fisher Scientific), while SK-MES-1 cells were in m ....