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Automated extraction of plasmid DNA

In this article, Analytik Jena details how to achieve faster, more reliable automated extraction of plasmidDNA. ....

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Ultra PCR Product Clean-Up Kit from Thermo Fisher Scientific

Read independent reviews on Ultra PCR Product Clean-Up Kit from Thermo Fisher Scientific on SelectScience ....

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Participation of GLO1 in the drug resistance of E. coli

To screen the potent genes associated with ESBLs in drug-resistant Escherichia coli, and elucidate the specific drug-resistant mechanism. ....

United States , Waals Gewest , Kit Shanghai Furi Technology Co Ltd , Clinical Laboratory Standardization Institute , Shanghai Biotechnology Co Ltd , Donglian Haer Instrument Manufacture Limited Company , Agilent Technologies , Takara Bio Inc , Selection Beads Yeasen Biotechnologies Ltd , Library Prep Kit For Illumina , Second Hospital Of Jilin University , Jk Chemical Ltd , Bacterial Specimen Collection , Second Hospital , Jilin University , Human Related Specimens , Ethics Committee , Bacteria Genomic , Transgen Biotech , Elution Buffer , Yeasen Biotechnologies Ltd , Prep Kit , New England Biolabs , Thermo Qubit , Genome Sequencing , Confirm Differently Expressed Genes ,

Cardiac cell type–specific gene regulatory programs and disease risk association

Misregulated gene expression in human hearts can result in cardiovascular diseases that are leading causes of mortality worldwide. However, the limited information on the genomic location of candidate cis-regulatory elements (cCREs) such as enhancers and promoters in distinct cardiac cell types has restricted the understanding of these diseases. Here, we defined >287,000 cCREs in the four chambers of the human heart at single-cell resolution, which revealed cCREs and candidate transcription factors associated with cardiac cell types in a region-dependent manner and during heart failure. We further found cardiovascular disease–associated genetic variants enriched within these cCREs including 38 candidate causal atrial fibrillation variants localized to cardiomyocyte cCREs. Additional functional studies revealed that two of these variants affect a cCRE controlling KCNH2/HERG expression and action potential repolarization. Overall, this atlas of human cardiac cCREs provides the found ....

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