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Antimicrobial Agents Of Future

Antimicrobial Agents Of Future
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France General , Elena Vialetto , Institut Pasteur , Nucleic Acids Research , Braunschweig Helmholtz Centre For Infection Research , Infection Research , University Of Toronto , Helmholtz Institute , Synthetic Biology Department , Tel Aviv University , Nucleic Acids , Braunschweig Helmholtz Centre , Chase Beisel , Different Strain , Acids Research ,

Researchers systematically investigate efficacy of CRISPR antimicrobial agents

Researchers systematically investigate efficacy of CRISPR antimicrobial agents
phys.org - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from phys.org Daily Mail and Mail on Sunday newspapers.

France General , Elena Vialetto , Institut Pasteur , Tel Aviv University , Helmholtz Institute , University Of Toronto , Synthetic Biology Department , Infection Research , Nucleic Acids Research , Braunschweig Helmholtz Center , Nucleic Acids , Chase Beisel ,

Noncanonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9


8). The tracrRNA hybridizes to the “repeat” portion of each crRNA within the transcribed array. Host-derived RNase III then cleaves the formed RNA stem to generate a processed crRNA:tracrRNA duplex utilized by Cas9 (
6). What remains unclear is whether crRNAs are confined to CRISPR-Cas loci or can be derived from elsewhere in the genome. Here, we show that crRNAs can be derived from host RNAs outside the CRISPR-Cas locus, inspiring a novel Cas9-based diagnostic platform that allows scalable detection of multiple biomarkers in a single test.
Cellular RNAs bound to Cas9 from
C. jejuni resemble crRNAs
C. jejuni NCTC11168 (CjeCas9) revealed crRNA-guided RNA targeting by CjeCas9 ( ....

Tatjana Achmedov , Patricia Guerry , Jiaqi Yu , Victor Corman , Rogier Louwen , Ioannis Mougiakos , Elena Vialetto , Daphne Collias , Lena Zubrod , Neva Caliskan , Christina Homberger , Peter Fineran , Interdisciplinary Center , Clinical Research , Leveraging Engineered , Creative Commons Attribution , Deutsche Forschungsgemeinschaft , Formal Analysis , Original Draft , Funding Acquisition , வெற்றியாளர் கார்மன் , இடைநிலை மையம் , மருத்துவ ஆராய்ச்சி , முறையான பகுப்பாய்வு , ஒரிஜிநல் வரைவு , நிதி கையகப்படுத்தல் ,