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[Full text] LINC00184 promotes OC proliferation and cisplatin resistance

Yuwen Han, Jun You, Yun Han, Yinglei Liu, Menghui Huang, Xiaoyan Lu, Jingjing Chen, Yanli Zheng Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, People’s Republic of China Correspondence: Yanli Zheng Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nantong University, No. 6 North Hai-Er-Xiang Road, Chongchuan District, Nantong, 226001, Jiangsu Province, People’s Republic of China Tel +86-0513-85061000 Objective: Cisplatin resistance is one of the main reasons for treatment failure in ovarian cancer (OC). Here, the effects of LINC00184 on cisplatin-resistant OC were studied. Patients and Methods: LINC00184, miR-1305 and CNTN1 expression in tissues from 70 OC patients was determined by qRT-PCR, in situ hybridization and Western blot. OC cell lines and OC cisplatin-resistant cell lines were cultured. Cells were transfected using Lipofectamine 2000 and treated with 100 nM

[Full text] Expression and Functional Relevance of ANXA1 in Hypopharyngeal Carcino

Cell Proliferation Assay The cell proliferation reagent CCK-8 (Monmouth Junction, NJ, USA) was used to assess the proliferation of FaDu cells. In short, cells transfected as described above and incubated for 48 h were added to 96 well plates (1×10 3 cells/well) containing 10% FBS medium. Ten microliters (10 μL) of CCK-8 were added to cells and cells were incubated at 37°C for 1 h. Finally, the optical density at 450 nm was measured using a microplate reader (iMark Microplate Absorbance Reader; Bio-Rad Laboratories, Inc., Hercules, CA, USA). Colony Formation Analysis The colony-forming assay was used to detect cell proliferation. Forty-eight hours (48 h) after transfection, 1000 cells per space were inoculated into six empty plates and incubated in medium containing 10% FBS for about 2 weeks. The medium was changed every three days. Finally, cells were fixed with 100% formaldehyde and stained with 0.5% crystal violet. The number of colonies was counted under an inverted microsc