2S
3 modified by oleic acid and PLGA-PEG-FA were obtained from Xi’an Ruixi Biological Technology Co., Ltd. (China). Perfluoropentane (PFP) was obtained from Strem Chemicals, Inc. (America). Cell Counting Kit-8 (CCK-8) was obtained from Dojindo (Japan). The 2-(4-Amidinophenyl)-6-indolecarbamidinedihydrochloride (DAPI) and Trypsin-EDTA solution (0.25% trypsin) were obtained from Beyotime Technology. Dichloromethane (CHCl
2) and isopropyl alcohol were obtained from Chongqing Chuan Dong Chemical Co., Ltd. (China). Poly (vinyl alcohol) (PVA), 1,1′-dioctadecyl-3,3,3′,3′- tetramethylindotricarbocyanine iodide (DiR) and Dialysis membrane (10000–12000Da) were obtained from Sigma-Aldrich Chemical Co., Ltd. (MW: 25000, America). McCoy’s 5A was obtained from Procell Life Science&Technology Co., Ltd.
8 Cytokines, including Il-1β, TNF-α, and PEG
2 and VEGF, have been certified to play an important role in DED, and by inhibiting expression of those cytokines, symptoms of DED could be significantly alleviated.
35–39 Traditional anti-inflammatory agents like corticosteroids would result in disastrous incidence if inappropriately applied, including intraocular hypertension and cataract,
10 which narrows its use in DED therapy. Therefore, an effective anti-inflammation agent with less impact on intraocular pressure is worthy of exploration. Tet, a plant alkaloid isolated from a traditional Chinese herb,
11 is initially used as an anti-inflammation agent to treat rheumatoid arthritis. It has been demonstrated that Tet could suppress the release of IL-1β, TNF-α, VEGF and PEG