It was not immediately clear whether any of the individuals have attorneys.
A CHP officer spotted the pair in a blue Acura Integra that was the subject of a be-on-the-lookout alert while heading south on Interstate 805 over Interstate 8, CHP said.
The driver of the car refused to yield and fled through southern San Diego, National City and Chula Vista before pulling over on Camino De La Plaza at Interstate 5, just north of the border, CHP public affairs Officer Salvador Castro said.
The brothers then jumped out of the vehicle and ran off. Personnel with the CHP and U.S. Customs and Border Protection chased after them, and took them into custody a short distance away, Castro said.