Reversed-phase liquid chromatography (RPLC) is often associated with quantitative or analytical-scale analysis, but it is also a useful preparative-scale tool for
purification. For any purification project, there are three possible objectives: purity,
yield, and throughput. These objectives are related and are commonly represented
as a triangle. Two of these targets can be achieved at the
expense of the third. Prioritizing the objectives of the purification project before
developing the met
Reversed-phase liquid chromatography (RPLC) is often associated with quantitative or analytical-scale analysis, but it is also a useful preparative scale tool for
purification. For any purification project, there are three possible objectives: purity,
yield, and throughput. These objectives are related and are commonly represented
as a triangle. Two of these targets can be achieved at the
expense of the third. Prioritizing the objectives of the purification project before
developing the met
Applications of Chromatography in COVID-19
The Sars-CoV-2 virus has caused a pandemic which has led to nearly 100 million cases globally by the end of 2020. The development of effective vaccines and treatments by companies and governments worldwide has gathered pace at an unprecedented speed, and robust analytical methods have been employed by researchers worldwide. This article will discuss the role chromatography techniques have played in the development of our response to the global pandemic.
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Chromatography
First devised by Mikhail Tsivet in 1900, chromatography is used to separate compounds within a mixture. It involves two phases, a mobile and a stationary phase. In the mobile phase, the mixture of interest is dissolved in a fluid, which can be either a gas, solvent, or water. This is then carried through a system where a material, or the stationary phase, is affixed.