Reversed-phase liquid chromatography (RPLC) is often associated with quantitative or analytical-scale analysis, but it is also a useful preparative-scale tool for
purification. For any purification project, there are three possible objectives: purity,
yield, and throughput. These objectives are related and are commonly represented
as a triangle. Two of these targets can be achieved at the
expense of the third. Prioritizing the objectives of the purification project before
developing the met