CDK1,
BUB1) play important roles in the progression of G2/M phase in the cell cycle.
30,31 Furthermore, real-time PCR analysis was used to verify the results of transcriptome sequencing and the results indicated that the expression trends of the top 18 ranked degree genes were consistent with those obtained by RNA-seq, suggesting that the RNA-seq data reliably reflected the gene expression alterations (Figure 4B). According to the RNA-seq and real-time PCR results, the expressions of cell cycle regulated genes, such as
CDK1,
PLK1, and
BUB1, were significantly decreased after sinomenine treatment. These results were also in accord with the cell phenotype experiments.
January issue of
SLAS (Society for Laboratory Automation and Screening)
Oak Brook, IL - The January edition of
SLAS Discovery features the cover article, Cryo-EM: The Resolution Revolution and Drug Discovery by Taiana Maia de Oliveira, Ph.D., Lotte van Beek, Ph.D., Fiona Shilliday, Ph.D., Judit E. Debreczeni, Ph.D., and Chris Phillips, Ph.D., from AstraZeneca.
Cryo-EM, a fast-growing technique in the pharmaceutical industry, enables structure determination of sought-after targets such as large multiprotein complexes and membrane proteins that eluded other structural biology methods. The intricate interactions between compounds and proteins are crucial in accelerating the drug discovery process. The cover article reviews how the structural understanding gained through Cryo-EM is influencing drug discovery projects focused on TRP channels, GPCRs, ribosomes and other high value targets.