In the past three decades, methods for sequencing nucleic acids have improved significantly in speed and
accuracy. Despite the technological advances in sequencing, preparation of libraries for Next-generation
sequencing (NGS) remains a tedious process. In this application note, Beckman Coulter Life Sciences demonstrates that the automated processing on the Biomek NGeniuS Next
Generation Library Prep System is equivalent to the manual preparations at input concentration of
200 ng (g
Clinical Implications of a New DDX58 Pathogenic Variant That : Journal of the American Society of Nephrology lww.com - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from lww.com Daily Mail and Mail on Sunday newspapers.
Viral diseases of Paris spp. have become serious problems in cultivation of this group of important medicinal plants in China. In this study, eight viruses were identified in the diseased plants of Paris yunnanensis by high-throughput sequencing (HTS) and RT-PCR. These viruses include three novel viruses (two potyviruses and one nepovirus), Hippeastrum chlorotic ringspot virus (HCRV)，Lychnis mottle virus (LycMoV), Paris mosaic necrosis virus (PMNV), Paris virus 1 and pepper mild mottle virus. The three new viruses were tentatively named Paris potyvirus 3 (ParPV-3), Paris potyvirus 4 (ParPV-4), Paris nepovirus 1 (ParNV-1) and their complete genome sequences were determined. Sequence analyses showed ParPV-3 and ParPV-4 shared the highest amino acid (aa) sequence identities of 54.3% to each other and 53.0-57.8% to other known potyviruses. ParNV-1 had aa sequence identities of 28.8-63.7% at protease-polymerase (Pro-Pol) with other nepoviruses. Phylogenetic analyses further support that t