The mummified remains used for the new study, published online in the journal Communications Biology, come from a Neapolitan noble from the Renaissance period in Italy. Giovani d Avalos was 48 when he died in 1586, and thought to have suffered from chronic inflammation of the gallbladder due to gallstones. 🌎 Ancient Genome of E Coli Reconstructed Using Gallstone of A 16th Century Italian Mummy.