The mummified remains used for the new study, published online in the journal Communications Biology, come from a Neapolitan noble from the Renaissance period in Italy. Giovani d'Avalos was 48 when he died in 1586, and thought to have suffered from chronic inflammation of the gallbladder due to gallstones. 🌎 Ancient Genome of E Coli Reconstructed Using Gallstone of A 16th Century Italian Mummy.