Through the actuation of vibronic modes in cell-membrane-associated aminocyanines, using near-infrared light, a distinct type of molecular mechanical action can be exploited to rapidly kill cells by necrosis. Vibronic-driven action (VDA) is distinct from both photodynamic therapy and photothermal therapy as its mechanical effect on the cell membrane is not abrogated by inhibitors of reactive oxygen species and it does not induce thermal killing. Subpicosecond concerted whole-molecule vibrations of VDA-induced mechanical disruption can be achieved using very low concentrations (500 nM) of aminocyanines or low doses of light (12 J cm−2, 80 mW cm−2 for 2.5 min), resulting in complete eradication of human melanoma cells in vitro. Also, 50% tumour-free efficacy in mouse models for melanoma was achieved. The molecules that destroy cell membranes through VDA have been termed molecular jackhammers because they undergo concerted whole-molecule vib
In Raman spectroscopy, accurate and sensitive identification of chemicals and materials can be compromised by fluorescence from laser excitation of the target substance itself and/or interferents in the sample matrix. Click to read more.
Raman spectroscopy has become an essential tool for chemists, physicists, and biologists. By analyzing subtle molecular vibration modes, the technique enables high-sensitivity non-invasive structural studies of different water-soluble and membrane-bound biomolecules (DNA, RNA, proteins) and their interactions under physiological conditions.
In this article, Metrohm Middle East discusses how their portable Raman microscopy equipment can be utilized in the measurement of microplastics, which are becoming a pollutant of ever-greater concern.