Mar 03 2021 Read 607 Times
It’s fair to say that oil and water have a complicated relationship. While they famously don’t mix, it’s also true that when there are no intervention measures in place, oil will usually find a way to reach water.
When it does, it’s incredibly problematic from an environmental perspective. Whether as the result of an accidental spill or deliberate, malicious activity, oil entering waterways and filtering through to the watercourse causes widespread damage. Just one litre of oil – a relatively small amount – has the potential to contaminate as much as 1 million litres of water.
Understanding Absolute Stoichiometry of Oligomeric Protein Complexes Using SEC-MALS
Thought LeadersSheena D Arcy & Prithwijit SarkarD Arcy Group University of Texas
In this interview, Sheena D Arcy and Prithwijit Sarkar from the D Arcy Group in the Department of Chemistry and Biochemistry at the University of Texas at Dallas talks to News-Medical Life Sciences about using SEC-MALS to characterize the native oligomeric state of Nap proteins and their complexes.
What is SEC-MALS?
SEC-MALS is size-exclusion chromatography coupled to multi-angle light scattering. The SEC component is comprised of an HPLC or FPLC pump and SEC column plus a UV absorption detector. An aliquot of solution that typically contains a mixture of different protein species is injected and flows through the packed column.