Understanding Absolute Stoichiometry of Oligomeric Protein Complexes Using SEC-MALS
Thought LeadersSheena D Arcy & Prithwijit SarkarD Arcy Group University of Texas
In this interview, Sheena D Arcy and Prithwijit Sarkar from the D Arcy Group in the Department of Chemistry and Biochemistry at the University of Texas at Dallas talks to News-Medical Life Sciences about using SEC-MALS to characterize the native oligomeric state of Nap proteins and their complexes.
What is SEC-MALS?
SEC-MALS is size-exclusion chromatography coupled to multi-angle light scattering. The SEC component is comprised of an HPLC or FPLC pump and SEC column plus a UV absorption detector. An aliquot of solution that typically contains a mixture of different protein species is injected and flows through the packed column.